Review



pcat®3-enhancer vector  (Promega)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Promega pcat®3-enhancer vector
    Comparison of the dual reporter system with the conventional CAT reporter system. The same promoter regions used in the dual reporter vector system were re-cloned into the CAT reporter system, <t>pCAT®3-Enhancer</t> vector (A). Vectors harboring different lengths of the promoter region were cotransfected with pSV-β-Galactosidase vector. The transcriptional activities of each region were determined via Chloramphenicol acetyl transferase activity assays (B, C) after compensation with beta-galactosidase. The pCAT3 control vector without the promoter sequence (pCAT®3-Basic) and the one harboring the SV40 promoter (pCAT®3-Control) were used as negative and positive controls, respectively.
    Pcat®3 Enhancer Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcat®3-enhancer vector/product/Promega
    Average 90 stars, based on 1 article reviews
    pcat®3-enhancer vector - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "A New Reporter Vector System Based on Flow-Cytometry to Detect Promoter Activity"

    Article Title: A New Reporter Vector System Based on Flow-Cytometry to Detect Promoter Activity

    Journal: Immune Network

    doi: 10.4110/in.2009.9.6.243

    Comparison of the dual reporter system with the conventional CAT reporter system. The same promoter regions used in the dual reporter vector system were re-cloned into the CAT reporter system, pCAT®3-Enhancer vector (A). Vectors harboring different lengths of the promoter region were cotransfected with pSV-β-Galactosidase vector. The transcriptional activities of each region were determined via Chloramphenicol acetyl transferase activity assays (B, C) after compensation with beta-galactosidase. The pCAT3 control vector without the promoter sequence (pCAT®3-Basic) and the one harboring the SV40 promoter (pCAT®3-Control) were used as negative and positive controls, respectively.
    Figure Legend Snippet: Comparison of the dual reporter system with the conventional CAT reporter system. The same promoter regions used in the dual reporter vector system were re-cloned into the CAT reporter system, pCAT®3-Enhancer vector (A). Vectors harboring different lengths of the promoter region were cotransfected with pSV-β-Galactosidase vector. The transcriptional activities of each region were determined via Chloramphenicol acetyl transferase activity assays (B, C) after compensation with beta-galactosidase. The pCAT3 control vector without the promoter sequence (pCAT®3-Basic) and the one harboring the SV40 promoter (pCAT®3-Control) were used as negative and positive controls, respectively.

    Techniques Used: Plasmid Preparation, Clone Assay, Chloramphenicol Acetyltransferase Assay, Activity Assay, Sequencing



    Similar Products

    pcat*3-enhancer vector  (Thermo Fisher)
    90
    Thermo Fisher pcat*3-enhancer vector
    Pcat*3 Enhancer Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcat*3-enhancer vector/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    pcat*3-enhancer vector - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    pcat®3-enhancer vector  (Promega)
    90
    Promega pcat®3-enhancer vector
    Comparison of the dual reporter system with the conventional CAT reporter system. The same promoter regions used in the dual reporter vector system were re-cloned into the CAT reporter system, <t>pCAT®3-Enhancer</t> vector (A). Vectors harboring different lengths of the promoter region were cotransfected with pSV-β-Galactosidase vector. The transcriptional activities of each region were determined via Chloramphenicol acetyl transferase activity assays (B, C) after compensation with beta-galactosidase. The pCAT3 control vector without the promoter sequence (pCAT®3-Basic) and the one harboring the SV40 promoter (pCAT®3-Control) were used as negative and positive controls, respectively.
    Pcat®3 Enhancer Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcat®3-enhancer vector/product/Promega
    Average 90 stars, based on 1 article reviews
    pcat®3-enhancer vector - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    pcat 3 enhancer vector  (Promega)
    90
    Promega pcat 3 enhancer vector
    Comparison of the dual reporter system with the conventional CAT reporter system. The same promoter regions used in the dual reporter vector system were re-cloned into the CAT reporter system, <t>pCAT®3-Enhancer</t> vector (A). Vectors harboring different lengths of the promoter region were cotransfected with pSV-β-Galactosidase vector. The transcriptional activities of each region were determined via Chloramphenicol acetyl transferase activity assays (B, C) after compensation with beta-galactosidase. The pCAT3 control vector without the promoter sequence (pCAT®3-Basic) and the one harboring the SV40 promoter (pCAT®3-Control) were used as negative and positive controls, respectively.
    Pcat 3 Enhancer Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcat 3 enhancer vector/product/Promega
    Average 90 stars, based on 1 article reviews
    pcat 3 enhancer vector - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    pcat-3 enhancer vector  (Promega)
    90
    Promega pcat-3 enhancer vector
    Comparison of the dual reporter system with the conventional CAT reporter system. The same promoter regions used in the dual reporter vector system were re-cloned into the CAT reporter system, <t>pCAT®3-Enhancer</t> vector (A). Vectors harboring different lengths of the promoter region were cotransfected with pSV-β-Galactosidase vector. The transcriptional activities of each region were determined via Chloramphenicol acetyl transferase activity assays (B, C) after compensation with beta-galactosidase. The pCAT3 control vector without the promoter sequence (pCAT®3-Basic) and the one harboring the SV40 promoter (pCAT®3-Control) were used as negative and positive controls, respectively.
    Pcat 3 Enhancer Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcat-3 enhancer vector/product/Promega
    Average 90 stars, based on 1 article reviews
    pcat-3 enhancer vector - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Comparison of the dual reporter system with the conventional CAT reporter system. The same promoter regions used in the dual reporter vector system were re-cloned into the CAT reporter system, pCAT®3-Enhancer vector (A). Vectors harboring different lengths of the promoter region were cotransfected with pSV-β-Galactosidase vector. The transcriptional activities of each region were determined via Chloramphenicol acetyl transferase activity assays (B, C) after compensation with beta-galactosidase. The pCAT3 control vector without the promoter sequence (pCAT®3-Basic) and the one harboring the SV40 promoter (pCAT®3-Control) were used as negative and positive controls, respectively.

    Journal: Immune Network

    Article Title: A New Reporter Vector System Based on Flow-Cytometry to Detect Promoter Activity

    doi: 10.4110/in.2009.9.6.243

    Figure Lengend Snippet: Comparison of the dual reporter system with the conventional CAT reporter system. The same promoter regions used in the dual reporter vector system were re-cloned into the CAT reporter system, pCAT®3-Enhancer vector (A). Vectors harboring different lengths of the promoter region were cotransfected with pSV-β-Galactosidase vector. The transcriptional activities of each region were determined via Chloramphenicol acetyl transferase activity assays (B, C) after compensation with beta-galactosidase. The pCAT3 control vector without the promoter sequence (pCAT®3-Basic) and the one harboring the SV40 promoter (pCAT®3-Control) were used as negative and positive controls, respectively.

    Article Snippet: Several promoter regions used in the dual reporter vector were re-cloned into the CAT expression vector system pCAT®3-Enhancer vector (Promega, Madison, WI), in order to ascertain whether these regions evidenced similar transcription activity in the CAT assay system ( ).

    Techniques: Plasmid Preparation, Clone Assay, Chloramphenicol Acetyltransferase Assay, Activity Assay, Sequencing